The Arc/AP-2 interaction mediates synaptic transmission

Arc controls AMPAR endocytosis through a direct interaction with clathrin-adaptor protein 2. eNeuro. Copyright and reuse: The Warwick Research Archive Portal (WRAP) makes this work of researchers of the University of Warwick available open access under the following conditions. This article is made available under the Creative Commons Attribution 4.0 International license (CC BY 4.0) and may be reused according to the conditions of the license. For more details see: A note on versions: The version presented in WRAP is the published version, or, version of record, and may be cited as it appears here. Accepted manuscripts are peer-reviewed but have not been through the copyediting, formatting, or proofreading process. which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Alerts: Sign up at eneuro.org/alerts to receive customized email alerts when the fully formatted version of this article is published. 15 project, designed the experiments to address the importance of Arc/AP-2 regulating 16 AMPAR trafficking, prepared hippocampal cultures and transfections for the patch-17 clamp experiments and wrote the manuscript. LLPdS conceived and designed the 18 experiments to map the Arc/AP-2 interaction and its function in mammalian cells, 19 performed experiments, analyzed and interpreted the data and helped to write the 20 manuscript. LPdA performed and analyzed the GST pulldown and biotinylation 21 experiments. SCW performed the immunoprecipitation experiments. MJW performed 22 and analyzed the electrophysiological experiments. YCJ: performed experiments, 23 analyzed and interpreted the data. JM designed the strategy to knockdown AP-2, 24 performed the cloning of the shRNA and the production of the lentiviruses, 25 2 performed the modelling analysis of the Arc structure and contributed to the writing 26 of the manuscript.